Other Confocal Sites on the Net
with Images/Animations


This is a list of sites (that I have managed to find!) that have sample images and animations related to confocal microscopy. The information at these sites serves as a small example of the many applications of confocal microscopy in research.

For each site, I have included a LINK to the page that has the images/animations as well as a link to the related HOME page (if pertinent to confocal microscopy at that site.)

PLEASE NOTE: For each site, I have only listed the organization name and location; for personal credits, please refer to each specific Web page/link. These pages are not listed in any special order!

If anyone would like me to include a link to their site, or knows of any more sites with confocal images/animations, or if I have presented incorrect information about any of the sites listed here, please contact me!

Link Home Site Info. Brief Description
-- IUBio Archive, Indiana University, USA images related to Drosophila melanogaster
Advanced Microscopy and Imaging Laboratory, State University of New York, USA MPEG animations: maize pollen, watertree
The Center for Biomedical Imaging Technology (CBIT), University of Connecticut Health Center, USA Image and animation (in both QuickTime and MPEG formats) of growth cone filopodia studied with high resolution video enhanced differential interference contrast microscopy. Also be sure to check here for more images related to digital microscopy from some of the other research being done at this site.
Integrated Microscopy Resource (IMR), UW-Madison Molecular Biology, USA images: safrinine stained, whole-mount zebra fish embryo in the tail region; interphase 3t3 cell stained with rhodamine phalloidin (revealing actin cables) and an FITC double layered antibody revealing microtubules; 3t3 metaphase spindle stained for microtubules. Also be sure to check out an animation (available in QuickTime and MPEG formats) of a single focal plane over time of a c. elegans nematode embryo developing which can be found here.
Laboratory of Plant Taxonomy, Kochi University, Japan images of mosses and algae; MPEGs showing optical section series and 3-D animation of stoma of (Diphyscium satoi) Tuzibe.
Confocal Facility, University of Guelph, Canada images: Pollen grains of (Perskia); stalked glandular hairs of (Drosera); microtubules in cells of a radish root apex; dog ovum; dog ovum following in-vitro fertilization.
Confocal Microscope Facility, University of Manchester, England an image and MPEG animation of a mouse embryo stained with Rhodamine/Phalloidin.
-- The Dow Lab, University of Glasgow, Scotland images and QuickTime movies relating to tubule-specific enhancer trap lines: a subset of primary epithelial cells; secondary, or "stellate", cells -- in Drosophila melanogaster.
FishScope, Department of Zoology, University of Washington, Seattle, USA time-lapse recordings illustrating the morphogenesis of Zebrafish embryos (MPEG/QuickTime animations.) There is a link to the still Image gallery page: images of a forming notochord; zebrafish neurulae; goldfish epidermal cell.
-- The Bowerman Lab, University of Oregon, USA images and QuickTime movies related to a rotational series of a pharynx stained with the 3NB12 monoclonal antibody, which stains the surfaces of pharyngeal muscle cells pm3, pm4, pm5, and pm7. -- related to C. elgans.
Dept. of Pathology, University of Melbourne, Australia "Image of the Week"
-- Institute for Scientific Computation, Texas A&M University, USA images demonstrating 3-D reconstruction from optical sections. Included on the page are 3-D geometric models for download that are in a format (*.iv) which can be read by SceneViewer on an SGI workstation. A tutorial file describing the techniques used is also available for download.
Biosciences and Neuroscience Groups, Southampton University, UK (biosciences) images: embryo, cytoskeleton, volvox, diatoms, copepod; link to more images at this site (neuronal morphology): multiple neurons within a hippocampal slice; pyramidal neurone structure; dendritic spine detail; cytoskeletal/membrane protein interactions.
Resource for Molecular Cytogentics, UCSF/Lawrence Berkeley Laboratory, CA, USA image: 4 surface-rendered views through a 20 micron thick melanoma section labeled with a centromeric chromosome 7 probe and propidium iodide.
-- Dept. of Physics, KTH, Sweden images and MPEGs related to glomeruli from rat kidney.
-- German Cancer Research Center Division Histodiagnostic and Pathomorphological Documentation, Germany MPEG animation: a pseudo 3-D visualization of the modeled surface of a nucleus. Demonstration of mathematical modelling of confocal data.
-- The Agouron Institute, La Jolla, CA, USA Images and QuickTime Animations: 3-D reconstructions of cells microinjected with modified oligos; COS7 nuclei that were injected with FITC and rhodamine-labeled oligos. Also, do not miss this page for more QuickTime animations demonstrating a blind deconvolution algorithm that improves the resolution of linear structures in all viewing perspectives.
-- Department of Molecular Biophysics, Debye Instituut, Utrecht University, Netherlands A QuickTime movie, obtained by rendering a 3-D data stack obtained from a confocal laser scanning two-photon excited fluorescence microscope.
M.E.Mueller-Insitut for Microscopy Biocenter of the University, Basel, Switzerland A introduction to confocal microscopy with demonstration images
-- Cornea Service, Department of Ophthalmology, University of Iowa Hospitals and Clinics, USA images related to the use of confocal microscopy in cornea imaging and testing
Duke University Medical Center, Orthopaedic Bioengineering Laboratory, USA images and an MPEG animation related to deformation of chondrocytes.
-- UCLA Visualization Center, USA images and an MPEG animation related to Texas Red-NAPS binding in live LZR-fibroblasts.
Department of Medical Physiology, Texas A&M University Health Science Center, USA Videomicroscopy Library: a collection of MPEGs and QuickTime animations from departmental labs. Also be sure to check out the Digital Image Library which contains a number of images also related to digital microscopy.
-- College of Sciences and Mathematics (Zoology/Wildlife Sciences), Auburn University, Alabama, USA Veliger Larva Home Page: images and QuickTime movies relating to the peptidergic and monoaminergic components of the veliger nervous system.
Applied Precision Inc., Seattle, WA, USA sample images captured with the DeltaVision Microscope System (a wide field, optical sectioning microscope workstation that uses deconvolution techniques to yield high resolution images.)
best Systeme GmbH, Unterfoehringen, Germany sample images, animations and a VRML model generated using the Imaris 3-D imaging software. Browse the site through the home page to find a few additional images.
-- Dept. of Environmental & Plant Biology, Ohio University, USA Stereo pairs of plant nuclei during double fertilization, in pollen, and chromosomes in mitosis and meiosis.
MRC Laboratory for Molecular Cell Biology, University College London, UK Animated GIF: Upon target cell recognition, the lytic granules of CTLs (cytoxic T lymphocytes) polarise towards the target cells. The lytic granules are shown stained for granzyme A and the tubulin cytoskeletons of both CTLs and target are stained for tubulin.
The Sullivan lab, Department of Biology, University of California, Santa Cruz, USA MPEG movies constructed from time lapse images of living embryos following injection with fluorescently labelled protein.
-- Laboratory of John W. Sedat, Macromolecular Structure Group, University of California, San Francisco Images and movies of nuclei and chromosomes obtained using several methods of 3-D microscopy, including computerized wide-field deconvolution microscopy for multiwavelength fluorescence imaging of living cells, and electron tomography for three-dimensional imaging in the elctron microscope.
-- Mark Sussman, Ph.D., The Children's Hospital and Research Foundation, Division of Molecular Cardiovascular Biology, Cincinnati, Ohio At this site you can view images and movies generated from confocal microscopy data which examine the relationship between degeneration of myofibrillar structure and cardiomyopathy.

Compiled by: Lance Ladic