FIONA kernel test against Airy disc simulation
This is the result of a simulation of a single-point fluorophore emitter, using an Airy disc simulator program. Common settings for all of the tests include: Aperture 500nm, wavelength 510nm, Pixel spacing 70nm, 0.1 of the pixels hidden by the borders, 40000 fluorescent photons, 20 photons per count, 256 samples/pixel in X and Y. Test1: no Poisson noise, no dark current. Test2: with Poisson noise, no dark current. Test3: with Poisson noise, 1000 dark photons per pixel dark current.
Test 1: FIONA kernel
| Poisson Noise | Dark Current | Mean Radial Error | Mean X Error | Mean Y Error | Max Radial Error | X Bias | Y Bias | Estimates/Sec | True Log | Tracking Log | Images |
| nonoise | nodark |
0.0019494 | 0.00112207 | 0.00144554 | 0.00451355 | 0.000189574 | 0.000110656 | 44.1365
|
Log |
VST Log |
Images |
| noise | nodark |
0.0116194 | 0.00803434 | 0.00668287 | 0.026351 | 0.00107008 | 0.000510902 | 38.763
|
Log |
VST Log |
Images |
| noise | 1kphotons |
0.0158757 | 0.0112974 | 0.00885879 | 0.0292512 | 0.00139034 | 0.002416 | 37.1479
|
Log |
VST Log |
Images |
Example images follow:
 airy_nonoise_nodark.000.bmp |
 airy_noise_nodark.000.bmp |
 airy_noise_1kphotons.000.bmp |
Test 2: Symmetric kernel
| Poisson Noise | Dark Current | Mean Radial Error | Mean X Error | Mean Y Error | Max Radial Error | X Bias | Y Bias | Estimates/Sec | True Log | VST Log | Images |
| nonoise | nodark |
0.00215877 | 0.00132769 | 0.00146223 | 0.00589521 | -0.000418738 | 0.000510918 | 56.11
|
Log |
VST Log |
Images |
| noise | nodark |
0.0103172 | 0.00714351 | 0.00602611 | 0.0231526 | 0.000365705 | 0.000927197 | 52.9385
|
Log |
VST Log |
Images |
| noise | 1kphotons |
0.0147961 | 0.0103369 | 0.00828923 | 0.0287145 | 0.00049377 | 0.00252805 | 55.2306
|
Log |
VST Log |
Images |
Example images follow:
airy_nonoise_nodark.000.bmp |
airy_noise_nodark.000.bmp |
airy_noise_1kphotons.000.bmp |