Effect of Bead Size on Accuracy and Speed

The beads used for these tests are generated algorithmically. They are a mixture of dark-on-gray discs of various radius and dark-on-gray Gaussian spots of various standard deviation. The background is 75% brightness and the darkest spot on the disc or the Gaussian is 25%. There is no noise in these images. The disc samples are averaged over a uniform grid that has 100 times the resolution in X and Y of the final image. The Gaussians are averaged over a uniform grid that has 10 times the resolution in X and Y. This should make it possible to precisely localize the spots to 1/100th of a pixel. For all trials, the Video Spot Tracker precision setting is 0.001 and sample_spacing is 0.1.

Test 1: Gaussian Bead

Bead Size	Tracker Radius	Bead Type	Motion	Mean Radial Error	Mean X Error	Mean Y Error	Max Radial Error	X Bias	Y Bias	Estimates/Sec	True Log	VST Log	Images
9	22	gaussian	Circular	0.00199283	0.00120423	0.00136171	0.00491608	-0.000174858	-4.95083e-005	0.989784	Log	VST Log	Images
6	16	gaussian	Circular	0.00221857	0.00141848	0.00144794	0.0067036	-2.02083e-005	-0.000252992	1.81873	Log	VST Log	Images
3	10	gaussian	Circular	0.00248374	0.00160908	0.00159324	0.00560728	0.000150717	0.000145742	4.62765	Log	VST Log	Images

Example images follow:

gaussian_9_sym_circle.0_0.0000.bmp

gaussian_6_sym_circle.0_0.0000.bmp

gaussian_3_sym_circle.0_0.0000.bmp

Test 2: Disc Bead

Bead Size	Tracker Radius	Bead Type	Motion	Mean Radial Error	Mean X Error	Mean Y Error	Max Radial Error	X Bias	Y Bias	Estimates/Sec	True Log	VST Log	Images
9	22	disc	Circular	0.00794929	0.00520858	0.00480382	0.0150286	0.00465065	-0.00425711	0.939876	Log	VST Log	Images
6	16	disc	Circular	0.00855189	0.00562089	0.00510201	0.0179931	0.00453672	-0.00415131	1.7909	Log	VST Log	Images
3	10	disc	Circular	0.00953062	0.00592442	0.00603202	0.0180365	0.00434958	-0.00402925	4.61527	Log	VST Log	Images

Example images follow:

disc_9_sym_circle.0_0.0000.bmp

disc_6_sym_circle.0_0.0000.bmp

disc_3_sym_circle.0_0.0000.bmp