The beads used for these tests are generated algorithmically. They are a mixture of dark-on-gray discs of radius 10 and dark-on-gray Gaussian spots of standard deviation 10. The tracker radius for the disc is set to 10 pixels. The kernel radius for the cone and symmetric kernels is set to 12 pixels. The background intensity in each image is 75% of maximum brightness and the darkest spot on the disc or the Gaussian is 25%. There is no noise in these images. The disc samples are averaged over a uniform grid that has 100 times the resolution in X and Y of the final image. The Gaussians are averaged over a uniform grid that has 10 times the resolution in X and Y. This should make it possible to precisely localize the spots to 1/100th of a pixel. For all trials, the Video Spot Tracker precision setting is 0.001 and sample_spacing is 0.1.
Example images follow:
![]() disc_disc_circle.0_0.0000.bmp |
![]() disc_cone_circle.0_0.0000.bmp |
![]() disc_symmetric_circle.0_0.0000.bmp |
Example images follow:
![]() gaussian_disc_circle.0_0.0000.bmp |
![]() gaussian_cone_circle.0_0.0000.bmp |
![]() gaussian_symmetric_circle.0_0.0000.bmp |